Artikel Jurnal Biochemical Evaluation of Withania somnifera Root Powder on Adjuvant-Induced Arthritis in Rats

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Abstract : The present
investigation was carried out to evaluate the biochemical effect of Withania
somnifera linn. Solanaceae, commonly known as ashwagandha on adjuvant induced
arthritic rats. Results were compared to Indomethacin, a non steroidal
anti-inflammatory drug. Arthritis was induced by an intra dermal injection of
Complete Freund's Adjuvant (0.1 ml) into the right hind paw of Wistar albino
rats. Withania somnifera root powder (1000 mg / kg / day) and Indomethacin (3
mg / kg / day) were orally administered for 8 days (from 11th to 18th day) after
adjuvant injection. After the experimental period, all the animals were
sacrificed and serum, liver and spleen samples were collected for further
biochemical analysis. A significant increase in the activities of gluconeogenic
enzymes, tissue marker enzymes, blood glucose level, WBC, platelet count,
erythrocyte sedimentation rate, and acute phase proteins (hyaluronic acid,
fibrinogen and ceruloplasmin) was observed in adjuvant-induced arthritic rats,
whereas the activities of glycolytic enzymes, body weight, levels of
hemoglobin, RBC count, and packed cell volume were found to be decreased. These
biochemical alterations observed in arthritic animals were ameliorated
promised after the administration of Withania somnifera root powder (1000
mg / kg / b.wt) and Indomethacin (3 mg / kg / b.wt). Our results suggest that Withania
somnifera root powder is capable of rectifying the above biochemical changes in
adjuvant arthritis and it may prove to be useful in treating rheumatoid
arthritis.

Keywords : adjuvant-induced
arthritis; Indomethacin; non-steroidal anti-inflammatory drug; Withania
somnifera root powder

Author : Mahaboobkhan Rasool,
Palaninathan Varalakshmi

Journal Code: jpkedokterangg150367



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