Abstract : Ixora coccinea Linn.
(Rubiaceae) has mentioned in Ayurveda as Paranti and traditionally stems used
in inflammatory diseases like sprains, eczema, contusions and boils. Present study deals with evaluation of
anti-inflammatory and antioxidant activity of extracts of I.coccinea stem.
Anti-inflammatory activity was studied in vivo by carrageenan-induced paw edema
in rat and in vitro by the human red blood cell membrane stabilization method.
Total tannin and flavonoid content of extracts was determined by using the
Folin-Ciocalteu method and aluminum chloride method, respectively. Antioxidant
activity was evaluated by an in vitro assay involving nitric oxide scavenging,
hydrogen peroxide scavenging, 2,2-
diphenylpicrylhydrazyl (DPPH) radical scavenging, and ion chelating
activity. Chloroform extract showed significant reduction in carrageenan
induced paw edema (p <0.05) and protection of HRBC in hypotonic solution. Methanol extract contains more total tannin and flavonoid content as compared with petroleum ether and chloroform extract. All extracts showed concentration dependant free radical scavenging activity. Methanol extract and chloroform extract has been shown as a good antioxidant activity and due to this antioxidant nature might be responsible for its anti-inflammatory activity. These activities supports to use of I.capinea extract in traditional used in treatment of various inflammatory disaeses.
Keywords : Anti-inflammatory
activity; Antioxidant activity; Carrageenan-induced paw edema; HRBC; Ixora
Author : Surana A R, Wagh R D
Journal Code: jpfarmasigg170015